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KMID : 0616120160470020150
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 2 p.150 ~ p.157
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Anti-Inflammatory and Antioxidant Effects of Clam Worm Extract in Macrophage RAW264.7 Cells
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Kim Se-Woong
Sapkota Mahesh
Li Liang
Yang Ming
Park Chan-IL
Soh Yun-Jo
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Abstract
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Earth worm (Eisenia andrei) and clam worm (Perinereis linea) have been used as anti-bacterial and anti-inflammatory agent. However, it is unclear how they exerted their physiological effects in macrophages. In this experiment, the anti-inflammatory and antioxidant effects of clam worm extract (CWE) and earth worm extract (EWE) in RAW264.7 cells were examined by measuring MDA, catalase, SOD, GSH-Px and inflammatory cytokines (nitric oxide, iNOS, interleukin-1b and tumor necrosis factor-a). Treatment with CWE significantly increased the activities of catalase, SOD and GSH-Px in RAW264.7 cells and decreased the level of MDA. Interestingly, treatment with CWE induced more activities of SOD than EWE. In addition, CWE decreased NO production, iNOS, COX-2, TNF-a and IL-1b in RAW264.7. The EWE also decreased NO production and iNOS, but increased COX-2 and IL-1b suggesting that CWE could be better resources for anti-inflammatory and antioxidant agent than EWE. Taken together, these results indicate that CWE has the potential as a natural antioxidant and a therapeutic for inflammation-related diseases.
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KEYWORD
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Earth worm, Clam worm, Anti-inflammation, Antioxidant
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